Modeling of Inactivation of Biofilm Composing Bacteria with Low Intensity Electric Field: Prediction of Lowest Intensity and Mechanism

Sterilization using high-intensity electric fields is detrimental to health if safety inadequate, so it necessary study the possibility of sterilization low-intensity fields. This aims determine lowest field intensity and treatment time deactivate bacteria that make up biofilms explain mechanism inactivation. The samples were from Pseudomonas aeruginosa Staphylococcus epidermidis grown on catheter. modeling formula was developed Pockels effect Weibull distribution with a square pulse-shaped pulse width 50 ?s an 2.0-4.0 kV/ cm. results showed threshold for irreversible electroporation both occurred in 3.5 kV/cm 3.75 cm, respectively, where size type Gram influenced. Moreover, had when occurred. However, there reversible electroporation, reduction number not significant. Also, changes conductivity affected